Spectral library searching for peptide identification in proteomics
نویسندگان
چکیده
منابع مشابه
Building and searching tandem mass spectral libraries for peptide identification.
Spectral library searching is an emerging approach in peptide identifications from tandem mass spectra, a critical step in proteomic data analysis. Conceptually, the premise of this approach is that the tandem MS fragmentation pattern of a peptide under some fixed conditions is a reproducible fingerprint of that peptide, such that unknown spectra acquired under the same conditions can be identi...
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SUMMARY A MapReduce-based implementation called MR-MSPolygraph for parallelizing peptide identification from mass spectrometry data is presented. The underlying serial method, MSPolygraph, uses a novel hybrid approach to match an experimental spectrum against a combination of a protein sequence database and a spectral library. Our MapReduce implementation can run on any Hadoop cluster environme...
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Computational analysis of mass spectra remains the bottleneck in many proteomics experiments. SEQUEST was one of the earliest software packages to identify peptides from mass spectra by searching a database of known peptides. Though still popular, SEQUEST performs slowly. Crux and TurboSEQUEST have successfully sped up SEQUEST by adding a precomputed index to the search, but the demand for ever...
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متن کاملPeptide identification in whole-sample mass spectrometry proteomics.
Peptide identification for whole-sample mass spectrometry (MS) proteomics is in its infancy. While sophisticated tandem MS/MS instrumentation exists for accurate peptide identification after sample separation, there are few options for those who produce data from intact protein samples. We present a novel algorithm which uses available information from the literature and online protein database...
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ژورنال
عنوان ژورنال: Statistics and Its Interface
سال: 2012
ISSN: 1938-7989,1938-7997
DOI: 10.4310/sii.2012.v5.n1.a4